Sanger Sequencing

For plasmids, PCR products and the templates that need a little extra attention.

Routine verification. Experienced hands.

Sanger sequencing on our ABI 3730xl DNA analyzers combines a 96-capillary array with high optical sensitivity. From a single tube to a stack of plates, choose a workflow suited to your template.

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Flexible preparation

Send your template and primer pre-mixed or separately.

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Difficult templates

Specialized workflows for GC-rich DNA, repeats and secondary structures.

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Technical support

Discuss primer choice, PCR cleanup and challenging reads.

Find your fit

Different questions.
The right service.

Start with what you want to learn. Our services offer complementary ways to understand your samples.

What would you like to check?

Each approach answers a different question. Compare the strengths and planning considerations below.

Help me choose →

At a glance	Sanger SequencingTargeted sequence verification	Whole PlasmidNanopore Sequencing	Linear / AmpliconNanopore Sequencing	Fragment AnalysisMolecular Services
Your research goal	Verify an insert, a PCR product or a specific sequence region.	Confirm a complete plasmid construct, including insert and backbone.	Examine a longer PCR product or a defined linear DNA target.	Measure fragment sizes and compare assay-specific marker patterns.
Core strength	Clear base-by-base readout around a chosen primer. Useful for focused verification.	Long-read context across the construct supports sequence and structural verification.	Long reads help preserve sequence context across the target region.	Size-based resolution for applications such as microsatellites, RFLP and ITDs.
Results to explore	Chromatogram, base calls and quality information.	Consensus sequence, plasmid annotation and read statistics.	Consensus sequence, read-length and coverage information; analysis agreed for the project.	Electropherogram with fragment sizes and signal intensities.
Plan your sample	Choose a suitable primer and clean template. Broader coverage may require additional reactions.	Submit high-purity clonal plasmid DNA. A reference helps with construct comparison.	Provide cleaned products, expected sizes and primer information. PCR amplification does not preserve native DNA modifications.	Confirm assay design, labeling and size range. This measures fragment size rather than the full base sequence.
	Start an order →	Start an order →	Discuss your project →	Discuss your project →

Need both targeted verification and wider sequence context? Our team can help combine services. Pricing, turnaround and reporting depend on your samples and project scope.

Technology references

Capillary electrophoresis applications ↗Whole-plasmid sequencing workflow ↗

Data & files

Explore what the analysis can show you.

Inspect the four signal channels and review individual base calls.

Chromatogram

Compare a region of interest with your expected sequence.

SEQFORGE Sequence example

Your base calls

>example_sample_read

ATGCCGTAGCTAGGCT
CGATCGATCGTACGAT
GCTAGCTAGCGTACGT

Illustrative sequence excerpt · review alongside the chromatogram.

Sequence readout

Illustrative previews. Final report format and scope are confirmed for your project.

Find your service.

Choose the approach that fits your sample and question.

Service	Sample / preparation	Application
Plasmids / PCR products	Pre-mixed or separate	Routine sequence verification
Difficult templates	Confirm with our team	GC-rich DNA, repeats, hairpins
PCR purification	PCR products	Cleanup before sequencing
16S rRNA sequencing	Confirm with our team	16S sequence analysis
Clinical sequencing	Discuss workflow scope	CLIA/CAP-regulated workflows

Contact our team for current pricing, input requirements and turnaround.

Ready to send samples?

Choose pre-mixed or separate template and primer. Confirm concentrations, volume and primer selection for your application.

Start an order

Prepare

Confirm requirements for your service.

Label & send

Match sample IDs to containers and arrange delivery.

Review

Explore your results with application support.

Common questions

Need something more specific?

Talk to our team ↗

Can you work with difficult templates?

Discuss our difficult-template workflow for GC-rich regions, repetitive sequences, secondary structures and long homopolymers.

Do I need to send my own primer?

You can supply your own primer or select an appropriate universal primer. Confirm availability and suitability before preparing samples.

Is PCR cleanup available?

Enzymatic PCR cleanup removes unincorporated primers and dNTPs before sequencing. Ask our team whether your product needs cleanup.

Is local pickup available?

Pickup is available in select areas. Call (858) 492-0518 to confirm coverage, scheduling and packaging.

Explore Sanger services, platform, benefits, and workflow

01 / THE PLATFORM

High throughput.
Quality in every run.

Our ABI 3730xl platforms combine high optical sensitivity and uniformity across a 96-capillary array with advanced separation polymers to deliver high-quality sequencing results.

Our team works with the complexities of Sanger sequencing at every scale, supporting research-use-only (RUO) and CLIA/CAP-regulated workflows.

ABI 3730xlCapillary electrophoresis96 capillariesDesigned for high throughputTubes to platesFlexible submission scale

02 / TYPES OF SERVICE

What are you working on?

Choose an application to see its workflow and the next step for your sample.

YOUR TEMPLATE, YOUR FORMAT

Plasmids or PCR Products

Choose whether to submit your template and primer together or separately.

How will you send your template and primer? Pre-mixed Standard

Provide your template and primer pre-mixed in a single well or tube according to the sample submission guidelines.

Browse free universal primers (new tab)

Start a Sanger order More service details (new tab)

Sample preparation guide

03 / FEATURES & BENEFITS

Confidence, from sample to sequence.

Quality data, flexible workflows, and a team ready to help.

Quality

Data routinely yielding long reads and quality above Q20.

Difficult Templates

Proprietary workflows for challenging templates, including GC-rich sequences and difficult repeats.

Convenient

Local pickup available in select areas.

Confirm pickup availability

Quick Turnaround

Starting from next day. Confirm timing for your service and sample arrival.

Support

Skilled technical support to help you plan your sequencing project and discuss your results.

Online

A customer portal for placing orders and retrieving data.

Start an order online, then sign in to submit and view your results.

04 / FOLLOW YOUR SAMPLE

From template to trace.

Select a step to see what happens to your sample.

01 / PREPARE

Template Purification

Prepare a suitable template for sequencing. PCR cleanup removes unincorporated primers and dNTPs when required.

05 / HOW TO ORDER

Your next run starts here.

Review the requirements, add your samples online, then sign in when you are ready to submit.

Review sample requirements

Check the required preparation for your selected application.

Open sample preparation guide

Prepare your template and primer

For standard submissions, send them separately and provide your own primer or choose a free universal primer. For pre-mixed submissions, combine them according to the guidelines.

Arrange your order and delivery

Confirm pickup availability and turnaround with our team before sending.

Call (858) 492-0518

A TEAM BEHIND YOUR TRACE

A challenging template?
Let’s work through it.

Discuss your sample and find the right starting point.

(858) 492-0518Main / Customer supportContact our team