Universal primers
Universal primers are available from our laboratory. Contact our team for the current list and availability.
Sanger sequencing Sample preparation
SANGER SEQUENCING
Sample preparation guide
DNA and primer quantities, packaging, and delivery for Standard and Pre-mixed Sanger orders.
These quantities apply to Sanger sequencing. For Whole Plasmid or other services, view Nanopore services or contact our team for preparation requirements.
Confirm which quantities to use.
Both the listed quantities and 2× quantities are shown below. Confirm with the laboratory which set to prepare for your submission.
Ask our team01 / STANDARD
DNA and primer supplied separately
Submit DNA separately from any customer-supplied primer. In your order, select a universal primer or identify the primer you are providing. Label all containers to match the order.
Swipe the table sideways to see all quantities.
| Template type | DNA concentration | Listed DNA amount | 2× DNA amount |
|---|---|---|---|
| PCR product DNA | 5–20 ng/µL | 50 ng | 100 ng |
| Single-stranded plasmid | 50–100 ng/µL | 150 ng | 300 ng |
| Double-stranded plasmid | 50–200 ng/µL | 300 ng | 600 ng |
| BAC | 100–200 ng/µL | 2,000 ng | 4,000 ng |
Customer-supplied primer
- Primer concentration
- 10 pmol/µL
- Listed primer volume
- 5 µL
- 2× primer volume
- 10 µL
DNA amount is a mass in ng; primer volume is in µL. Use the quantities confirmed by the laboratory.
Start a Standard order02 / PRE-MIXED
DNA and primer supplied together
Identify the primer used for each mixture in your order. The tables show DNA amount, primer amount, and total mixture volume.
Use the set confirmed by the laboratory: listed quantities or 2× quantities.
Swipe the table sideways to see all quantities.
| Template type | DNA amount | Primer amount | Total volume |
|---|---|---|---|
| PCR product DNA | 50 ng | 10 pmol | 6 µL |
| Single-stranded plasmid | 150 ng | 10 pmol | 6 µL |
| Double-stranded plasmid | 300 ng | 10 pmol | 6 µL |
| BAC | 2,000 ng | 10 pmol | 6 µL |
Swipe the table sideways to see all quantities.
| Template type | 2× DNA amount | 2× primer amount | 2× total volume |
|---|---|---|---|
| PCR product DNA | 100 ng | 20 pmol | 12 µL |
| Single-stranded plasmid | 300 ng | 20 pmol | 12 µL |
| Double-stranded plasmid | 600 ng | 20 pmol | 12 µL |
| BAC | 4,000 ng | 20 pmol | 12 µL |
Primer amount in pmol is different from primer concentration in pmol/µL. The volumes above are the total volumes of the DNA–primer mixtures.
Start a Pre-mixed order03 / PRIMERS
Choose and identify your primers
Customer-supplied primers
Label each primer clearly and identify its corresponding samples in the order. For Pre-mixed submissions, identify the primer included in each mixture.
04 / PACKAGING
Label and package your samples
1.5 mL tubes with pop caps
Use 1.5 mL microcentrifuge tubes with pop caps, labeled to match the order form.
For orders of 24 samples or more, use 96-well plates instead of tube strips.
Normalize and seal
Normalize DNA and primer concentrations according to the applicable preparation requirements. Arrange samples vertically: A1–H1, then A2–H2, and continue by column.
Seal or cap the plate with strip lids to prevent leakage.
05 / ORDERING & DELIVERY
Submit your order and arrange delivery
Match the labels to the order
Check that each tube or plate position matches your sample list. Include your order form or a printed order confirmation with the samples.
Confirm the drop-off or pickup arrangement
Select a Dropbox or another available delivery method during checkout. Placement details and pickup schedules depend on the location; confirm the arrangement before sending your samples.
Find a Dropbox
Not sure which quantities to use?
Tell us your template type and submission format. Our team can confirm the preparation and delivery details.