Whole-construct context
Examine your insert alongside its surrounding plasmid sequence.
SeqForge services
Nanopore Sequencing
Whole Plasmid and Linear / Amplicon Sequencing for complete constructs and longer targets.
Start an order ↗Put your sequence in context.
Long reads connect more of your construct in a single read. Explore complete plasmids, verify engineered sequences or investigate a long target with project-specific analysis and scientific support.
Useful deliverables
Consensus sequences, read statistics and feature annotation.
Flexible analysis
Discuss reference comparison, variants and custom interpretation.
Find your fit
Different questions.
The right service.
Start with what you want to learn. Our services offer complementary ways to understand your samples.
Each approach answers a different question. Compare the strengths and planning considerations below.
Help me choose →| At a glance | Sanger SequencingTargeted sequence verification | Whole PlasmidNanopore Sequencing | Linear / AmpliconNanopore Sequencing | Fragment AnalysisMolecular Services |
|---|---|---|---|---|
| Your research goal | Verify an insert, a PCR product or a specific sequence region. | Confirm a complete plasmid construct, including insert and backbone. | Examine a longer PCR product or a defined linear DNA target. | Measure fragment sizes and compare assay-specific marker patterns. |
| Core strength | Clear base-by-base readout around a chosen primer. Useful for focused verification. | Long-read context across the construct supports sequence and structural verification. | Long reads help preserve sequence context across the target region. | Size-based resolution for applications such as microsatellites, RFLP and ITDs. |
| Results to explore | Chromatogram, base calls and quality information. | Consensus sequence, plasmid annotation and read statistics. | Consensus sequence, read-length and coverage information; analysis agreed for the project. | Electropherogram with fragment sizes and signal intensities. |
| Plan your sample | Choose a suitable primer and clean template. Broader coverage may require additional reactions. | Submit high-purity clonal plasmid DNA. A reference helps with construct comparison. | Provide cleaned products, expected sizes and primer information. PCR amplification does not preserve native DNA modifications. | Confirm assay design, labeling and size range. This measures fragment size rather than the full base sequence. |
| Start an order → | Start an order → | Discuss your project → | Discuss your project → |
Need both targeted verification and wider sequence context? Our team can help combine services. Pricing, turnaround and reporting depend on your samples and project scope.
Data & files
Explore what the analysis can show you.
Review the polished consensus sequence of your plasmid or target.
SEQFORGE FASTA example
Your consensus sequence
>example_plasmid_consensus
ATGCCGTAGCTAGGCT
CGATCGATCGTACGAT
GCTAGCTAGCGTACGT
ACGTAGCTAGCTAGGCIllustrative sequence excerpt · not experimental data.
Consensus sequence (.fasta)
Locate annotated features within the complete construct.
Plasmid map
Understand the distribution of reads supporting your analysis.
Read-length statistics
Review annotated features alongside the consensus sequence.
SEQFORGE Annotation example
Feature annotations
| Feature | Type | Position (bp) |
|---|---|---|
| Promoter | Regulatory | 101–250 |
| Insert | CDS | 301–1200 |
| Origin | Replication | 2201–2850 |
Illustrative features and coordinates.
Feature table
Request variant reporting when a suitable reference sequence is provided.
SEQFORGE Sequence example
Sequence comparison
REFERENCE
ATGCCTAGACGTACGAEXAMPLEATGCCTAGTCGTACGAIllustrative alignment · reference comparison is project-dependent.
Reference comparison
Illustrative previews. Final report format and scope are confirmed for your project.
Find your service.
Choose the approach that fits your sample and question.
| Service | Sample / preparation | Application |
|---|---|---|
| Whole Plasmid | Purified clonal plasmid DNA | Construct and structure verification |
| Linear / Amplicon | Purified products or pools | Full-length targeted regions |
| Custom analysis | Discuss your project | Reference comparison and interpretation |
Contact our team for current pricing, input requirements and turnaround.
Ready to send samples?
For plasmids, submit high-purity DNA in 10 mM Tris or nuclease-free water. For amplicons, remove primers and dimers and provide expected sizes and primer information.
Start an orderPrepare
Confirm requirements for your service.
Label & send
Match sample IDs to containers and arrange delivery.
Review
Explore your results with application support.
Whole plasmid or linear / amplicon?
Choose Whole Plasmid for purified circular constructs. Choose Linear / Amplicon for purified PCR products, amplicon pools or native linear DNA projects.
What files will I receive?
Whole plasmid projects include consensus FASTA, read-length statistics, a pLannotate plot and a feature annotation table. Reference-based variant reporting requires a reference and an agreed scope.
Can PCR products retain native modifications?
PCR does not preserve the original DNA’s native modification pattern. Modification analysis requires suitable PCR-free input and supported analysis methods.
Is local pickup available?
Pickup is available in select areas. Call (858) 492-0518 to confirm coverage, scheduling and packaging.
FROM SAMPLE TO INSIGHT
Long reads. A clearer whole.
Explore the workflow and discuss your project requirements with our team.
Submit purified DNA with matching sample labels, expected sizes and accurate quantification.
Whole Plasmid Sequencing — coverage, applications & files
Unlock the full potential of plasmid analysis.
Long reads help characterize complete plasmids and complex vectors, bringing sequence and structural context together. Real-time data acquisition and scalable workflows support projects from individual constructs to larger batches.
Use whole plasmid sequencing for synthetic construct validation, therapeutic-vector research, microbial plasmid studies and manufacturing research quality control. Consensus analysis can help investigate insertions, deletions and structural differences.
Delivered with your analysis
Consensus FASTA, read-length statistics, a pLannotate plasmid plot and an annotated feature table. Reference-based variant reporting is available when a reference is provided and included in the agreed scope.
Submit pure, double-stranded clonal plasmid DNA. Input quantity, achievable coverage, timing and pricing are confirmed for your construct and batch.
Start a whole plasmid order →Linear / Amplicon Sequencing — targets, applications & files
Keep your target in context.
Long-read sequencing can span full-length targets and preserve phase across variants. Discuss full-length 16S and ribosomal operons, viral targets, HLA loci, synthetic fragments, large gene regions and CRISPR validation with our team.
Submit purified PCR products or cleaned amplicon pools, free of primers and dimers. The supplied preparation guidance is 50–100 ng total, depending on pool complexity; confirm your project’s input requirement. Include expected sizes and primer sequences, or request design support.
Delivered with your analysis
Consensus FASTA per barcode, read-length and coverage plots, and consensus quality statistics. Reference alignment, variant analysis and phylogenetic placement are optional. Modification reports require an appropriate native, PCR-free workflow and supported targets.
Library preparation, multiplexing, sequencing depth and analysis are tailored to your project. Read length, turnaround and accuracy depend on input quality and agreed scope.
Discuss linear / amplicon sequencing →Expert support, flexible projects
SeqForge supports academic, biotechnology and pharmaceutical research with application planning and analysis support. Discuss construct comparison, mutation checks, project scale and data delivery with our team.
Talk to us · (858) 492-0518